ThreatLocker, a global player in Zero Trust cybersecurity, has raised $190m in a Series F round as it looks to counter the growing security risks posed by AI agents and machine-generated exploits.
The round was led by Elephant, with ongoing backing from D. E. Shaw Ventures and Arthur Ventures, alongside a substantial first-time commitment from Koch Disruptive Technologies.
The fresh capital will be channelled into deepening the firm’s controls for AI-related security risks, enhancing its Zero Trust Platform, and pushing into new international markets, starting with a new office in Reading in the UK.
The raise arrives at a moment when businesses must contend with a threat landscape transformed by the need to secure AI agents and repel software exploits accelerated by AI.
ThreatLocker has broadened its platform to address both conventional and AI-driven activity. Its Allowlisting capability blocks unauthorised AI tools and AI-generated code from running, while Ringfencing governs what sanctioned applications and AI agents are permitted to access, alter and interact with.
Further controls allow firms to manage employee use of AI websites and shield sensitive data from unauthorised exposure. These additions follow the launch of ThreatLocker Zero Trust Network Access and Zero Trust Cloud Access, which extend the platform across endpoints, networks and cloud resources through a single dashboard.
ThreatLocker specialises in prevention-first security built on a deny-by-default philosophy, meaning organisations define what is permitted and block everything else. The company argues this reverses the traditional allow-by-default posture of most security tooling, handing firms control over the software, users and devices operating inside their environments.
More than 70,000 organisations globally now use its platform, which the company has designed to be simple to deploy, manage and scale despite Zero Trust’s reputation for being hard to implement.
The business has grown its international footprint considerably over the last 18 months, opening offices in Brisbane and Dubai to complement existing bases in Orlando and Dublin. It has also featured on the Inc. 5000 list of America’s fastest-growing private companies for two years running.
As part of the deal, Koch Disruptive Technologies will draw on its partner network, sector expertise and Koch Labs capabilities to support the company’s growth.
ThreatLocker CEO and co-founder Danny Jenkins said, “Most cybersecurity tools are still built around identifying malicious activity after it has already entered an environment, when the damage may already be done. We believe the better model is to define what is allowed and deny everything else by default.
“We are living in a world where new agents are constantly being introduced and granted access to sensitive resources, which makes our mission more urgent. Our investors share our vision for changing the cybersecurity paradigm, and their partnership will help us strengthen our platform and protect more organizations around the world.”
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